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KMID : 0358419960390030572
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Korean Journal of Obstetrics and Gynecology
1996 Volume.39 No. 3 p.572 ~ p.588
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Pattern of p53 Gene Mutations and Human Papillomavirus Infections in Small Cell Carcinoma of the uterine Cervix
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Abstract
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Small cell carcinoma of the uterine cervix is a rare and aggressive tumor with poor prognosis. Several studies have shown that small cell carcinoma of the uterune cervix is associated with HPV 18 infection and alteration of p53 gene. The purpose
of
this
study is to analyse the relationship between clinical profiles, HPV infection and p53 alterations in small cell carcinoma of the uterine cervix.
Paraffin embedded tissue sections were obtained from 21 patients with small cell carcinoma of the cervix. The presence of HPV DNA sequences were detected by polymerase chain reaction(PCR) and Southern blotting. Alteration of p53 activity were
evaluated
by immunostaining using MAb DO7, and p53 mutation were evaluated by single strand conformation polymorphism(SSCP) and automatic sequencing of PCR products.
HPV 16 and HPV 18 DNA were detected in 20 of 21(95.2%) tissue samples, and HPV 18 DNA was detected in 9 of 21(42.8%) tissue samples. The alteration of p53 protein were founded in 6 of 21(28.5%) patients by immunohistochemical staining. Four of
these 6
sho wed anabnormal band shift at SSCP. One of these 4 cases were found to have a missense mutation by automatic sequencing.
Small cell carcinomas of the uterine cervix may have the same pattern of HPV infection and p53 alteration as large cell type carcinoma of the uterine cervix.
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KEYWORD
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